Epidermal Resident Memory T Cell Fitness Requires Antigen Encounter in the Skin

Avatar
Poster
Voice is AI-generated
Connected to paperThis paper is a preprint and has not been certified by peer review

Epidermal Resident Memory T Cell Fitness Requires Antigen Encounter in the Skin

Authors

Weiss, E. S.; Hirai, T.; Li, H.; Liu, A.; Baker, S.; Magill, I.; Gillis, J.; Zhang, Y. R.; Ramcke, T.; Kurihara, K.; The ImmGen Consortium OpenSource T cell Project, ; Masopust, D.; Anandasabapathy, N.; Singh, H.; Zemmour, D.; Mackay, L. K.; Kaplan, D. H.

Abstract

CD8+ tissue resident memory T cells (TRM) develop from effectors that seed peripheral tissues where they persist providing defense against subsequent challenges. TRM persistence requires autocrine TGF{beta} transactivated by integrins expressed on keratinocytes. TRM precursors that encounter antigen in the epidermis during development outcompete bystander TRM for TGF{beta} resulting in enhanced persistence. ScRNA-seq analysis of epidermal TRM revealed that local antigen experience in the skin resulted in an enhanced differentiation signature in comparison with bystanders. Upon recall, TRM displayed greater proliferation dictated by affinity of antigen experienced during epidermal development. Finally, local antigen experienced TRM differentially expressed TGF{beta}RIII, which increases avidity of the TGF{beta}RI/II receptor complex for TGF{beta}. Selective ablation of Tgfbr3 reduced local antigen experienced TRM capacity to persist, rendering them phenotypically like bystander TRM. Thus, antigen driven TCR signaling in the epidermis during TRM differentiation results in a lower TGF{beta} requirement for persistence and increased proliferative capacity that together enhance epidermal TRM fitness.

Follow Us on

0 comments

Add comment