Soomro, N. I.; Bakhtiar, S. M.

This study aims to investigate the role of impaired insulin resistance in the onset and progression of metabolic diseases such as prediabetes, diabetes, and cardiovascular diseases. Insulin resistance occurs when insulin is unable to effectively stimulate glucose uptake, and if the body is unable to produce sufficient insulin to compensate, type 2 diabetes may develop. This research endeavors to elucidate the molecular and genetic underpinnings of insulin resistance and its association with metabolic disorders. Employing various tools and databases, gene interaction data was procured through GeneMania, and pathway validation was conducted using KEGG. Construction of gene regulatory networks employed GEPHI 0.9.2, with centralities statistical analysis identifying hub genes. Enrichment analysis and literature validation substantiated the significance of these hubs, resulting in the refinement of the initially identified seven genes to five with interaction data. The implicated hub genes were discerned to play roles in inflammation, either directly or indirectly. Future prospects involve further genetic analysis across diverse populations, utilizing PCR to discern the allelic variations of these identified hub genes. Ultimately, this research may shed light on the underlying genetic and molecular mechanisms of insulin resistance and metabolic syndrome, and contribute to the development of targeted treatments for these conditions.